The bacterial biofilm formed on the surface of traditional medical devices during use is easy to cause infection and increase the incidence rate.The "silo" shaped bacteriostatic microstructure, which was processed by femtosecond laser two-photon polymerization (TPP) technology in two-photonic materials doped with dihydromyricetin (DMY),was proposed. In this paper,the effective incorporation of DMY in two-photonic materials was determined by electron circular dichroicsm (ECD) spectroscopy,and the optimal range of processing parameters for doped two-photonic materials is determined:printing speed of 10 μm/s—100 μm/s,and laser power of 18.1 mW—90.0 mW.A "silo" shaped bacteriostatic microstructure with a height of 30 μm was further prepared, and the intensive stability of it was attributed to the increased number of microstructure contour scans.This work has reference significance for the preparation and further development of microstructures with high sterilization rate.