In order to reduce the complexity of r adio over fiber(RoF) system and improve the frequency multiplication factor,a ne w scheme to generate frequency 16-tupling millimeter-wave based on polarizatio n multiplexing technology and cascaded Mach-Zehnder modulators (MZM) was propos ed.The millimeter-wave generation mechanism was deduced,and the effects of MZM extinction ratio,RF driving signal phase shift,polarization controller (PC) azim uth and linear polarizer (Pol) angle on optical sideband suppression ratio (OSSR ) and radio frequency sideband suppression ratio (RFSSR) were discussed.The perf ormance of ROF system based on the proposed scheme was studied.The results show that the high performance millimeter-wave signal can be generated in a wide off set range by controlling the parameters offset reasonably,and the corresponding optimal OSSR and RFSSR are 30dB and 22.6dB respectively.The power penalty of 40km transmission in optical fiber is 2.4dB compared with back-to-back transm ission at the bit error rate (RBE) of 10－9,and th e corresponding optical power budget is 50dB.The scheme is simple in structure and efficient in performance.The wavelength dependent devices such as optical fi lter are not used in the proposed system,which is compatible with the existing W DM passive optical network,and also provides reference for future ROF system des ign.