In this study,anthracene was selected as the research object,and its laser-induced fluorescence emission spectral characteristics in different solid and liquid phases of soil were studied.The results show that the fluorescence peaks for anthracen e in soil are at 420nm and 444nm induced by a 266nm laser.When the proportions of primary and secondary minerals in the soil changed,the fluorescence spectral peak positions of anthracene did not shift significantly,a nd relative standard deviation (RSD) of the fluorescence intensity of anthracene in 420nm was 4.52%.However,when the speci es and concentration of humus in the soil changed,the fluorescence intensity of anthracene decreased with increa sing concentration.The RSDs of fluorescence intensity of anthracene with different concentrations of humic acid and fulvic acid were 38.1% and 27.4% respectively at 420nm.Moreover,the results of range analysis of orthogonal exp eriments show that the effect of humic acid was greater than that of fulvic acid.In liquid-phase heavy metals c ontaminated soil,the fluorescence intensity of anthracene at 420nm decreased significantly,and showed little difference in soils with different concentrations of heavy metals in liqu id phase,and the positions of the fluorescence peak did not shift significantly.When the concentration of Cd was different,the RSD was 1.60%.When the concentration of Cu was different,the RSD w as 2.05%.When the concentration of Pb was different,the RSD was 4.54%.Therefore,when using the fluorescence peak intensity calibration method,the difference in spectral inten sity caused by changes in the solid-liquid composition should be considered.The obtained results provid the basic resear ch data for accurate measurement of anthracene in soil by using laser-induced fluorescence spectroscopy.