Gold (Au) metallic nanostructures were fabricated by self-assembling gold nanoparticles (GNPs) on 3-Aminopropyltrimethoxysilane (APS) modified glass sub strate.Surface enhanced fluorescence is studied for Rhodamine 6G molecules deposited on the fabricated substrates.The experimen tal results show that the prepared substrate presents strong fluorescence enh ancement,and the enhanced factor is about 4.2-fold with 532nm excited laser beam.Higher intensity of lo cal electric field and stronger coupling of surface plasmon resonance in gold nanostructure can enhance molecules more effectively.The results suggest that the current fabricated metallic substrate c an enhance electron transition,and can produce good fluorescence enhancement.