Development of quantitative and non-i nvasive methods for determination of optical parameters of turbid media can have wide applications in material analysis and medicine.We present a novel method t o acquire multispectral and high dynamic range reflectance images that can be compared wit h simulated image data to investigate the dependence of the image contrast on the optical properties of heterogeneous turbid samples.At each wavelength, the distribution of reflected light intensity at the surface of a turbid phantom is acquired from a camera with 4different exposure times which are used to determine a high dynamic range refl ection image.The incident beam profile is measured with a diffuse reflectance standard to obtain a reflectance image at each wavelength.The image of the incident beam profile is also imported into a Monte Carlo model to accura tely obtai n the simulated reflectance images within the framework of radiative transfer theory.We have measured refle ctance images of 5heterogeneous turbid samples in the spectral range from 500nm to 940nm.By accurate measurem ent of reflectance image with a full-field illumination using a diffuse reflectance standard and image simulati on with the Monte Carlo method,we demonstrate the feasibility to obtain the optical parameters of the turbid samp les with the measurement and simulation methods in a wide spectral region from 500nm and 940nm.