A kind of multi-function landmark,which is used for vision-based robot localiz ation and navigation,is designed in this paper.By optimizing the topological str ucture,the geometrical structure and the color selection,the designed landmark of this paper not only could provide plenty of information,but also has anti-interference ability.The designed landmark can locate and navigate the ro bot based on a single image using only one landmark.It can also navigate the robot in a large area using a series of landmarks in order to reduce the accumulative error.The position of each landmark of the series can be estimated accurately so as to improve the efficiency of navigation.Experiments in disturbing environments sho wn that even based on fish eye lens without camera rectification,using the designed landmark,the maximum error of robot localization is within 5cm, and the maximum error of teaching and playback navigation is within 10cm.Both t he localization error and the navigation error are not accumulative.All these can verify the efficiency of the landmark.