In this paper,a confocal laser scanning microscope coupled wi th a time-correlated single photon counting (TCSPC) module is used to measure the fluorescence anisotropy of the Rhodamine B glycerol and sugar solution with various concentrations.The experimental results show that fluorescence polarization and viscosity increase with the concentration increas ing.Moreover,lifetime is inversely proportional to the viscosity in the solution.Based on the results of our experiment,we present th e numerical relationship between the rotational relaxation time and the rotational correlation time.The basic principle of fluo rescence anisotropy analysis technology and relevant theories are introduced.Time-resolved fluorescence anisotropy imaging c an quantitatively measure the anisotropy of the sample microenvironment.We finally predict the viscosity sensitivity in live cells and indicate its future directions and applications.