Real-time micro-vibration measurement is widely used in engineering applications.It is very difficult for traditional optical detection methods to achieve real-time need in a relatively high frequency and multi-spot synchronous measurement of a region at the same time,especially at the nanoscale.Based on digital holographic interferometry t heories,a method of real-time micro-vibration multi-spot synchronous measurement of a region is proposed to satisfy the demand in enginee ring applications.A corresponding measuring system is built according to this approach.The high speed acquired holograms are localy c a lculated.Vibration information of the region can be extracted directly and instantaneously from the holograms by using a fast demodulation alg orithm.By this way,the measuring range is enlarged remarkably.The proposed method can achieve real-time need in a relatively high frequency and multi-spot synchronous measurement of a region at the same time.The feasibility of the system is demonstrated by measur ing the vibration of a headset diaphragm calibrated by the high precision Doppler vibration measuring instrument.The results demonstrate that t he system is feasible,when the frequency range of the vibration is between 100Hz and 2400Hz.The measurement error is 3.7% on average and every point in the measurement region has the same accuracy.