In this paper,a more widely suitable Otsu thresholding method is proposed based on cross division of two-dimensional histogram.The region is divided by two lines both via thresholding vector point.The angle between line one and gray level axis is α and the angle between line two and gray level axis is β,then more ordin ary regional division can be acquired.Traditional Otsu thresholding method is regarded as a special case of this method when α=0°,β=90°,while Otsu′s curve thresholding segmentation method as a special case when α=β=135°, Otsu′s θ-division thresholding segmentation method as a special case when 90°＜α=β＜180°.This improved two-dimensional histogram cross division method also has a wide application in other thresholding methods, such as maximum entropy which is based on two-dimensional histogram.The experiment results show that the proposed method not only achieves more accurate segmentation results and more robust anti-noise performance,but also has efficient and more practical value in applications.