The pulse wave is measured in radial artery based on a single reflective photo-electric probe.The single-chip micro-computer ATmega 16 is used to control LED with 660 nm and 940 nm wavelengths and to obtain and analyze the pulse wave.The blood oxygen saturation and heart rate are obtained by reading out the position of the extremum of the pulse wave,and then the respiration rate is obtained by processing the pulse wave with radical 2 time extraction fast fourier transform(FFT).10 volunteers are recruited to perform the physiological parameters measurement.We analyze the correlation between the results measured by the designed system and the data recorded in real time.The correlation coefficients of the blood oxygen saturation,heart rate and respiration rate are 0.985,0.982 and 0.933,respectively.It can be found that the physiological parameters measured by the designed system and the recorded date in real time have a good agreement and we will realize the multi-physiological parameters measurement noninvasive for long period by this system.