This paper studies the laser′s effect on the seed of Erigeron breviscapus(Vant.) Hand.-Mazz.The He-Ne laser and CO2 laser are used as the lamp-house to irradiate the seed,and the changes of the compounds in the seed are detected by UV-Vis spectrophotometer and high performance liquid chromatography(HPLC).The results show that the laser can influence the compounds in the seed.The influence of the short irradiated time is more obvious than the long irradiated time,and the effect of CO2 laser is more obvious than He-Ne laser.The content of scutellarin is higher than others when using CO2 laser for 0.5 min.So the Erigeron breviscapus(Vant.) Hand.-Mazz.may produce breeding by laser.