Calibrating both the location and the shape of structured light in 3-D measurement system based on multi-point fitting method has been presented. In the field of view,the target plane has been rotated and shifted for times to cover the entire efficient measurement range. Then,an adequate number of 3-D points of each stripe intersected on the target plane in different positions have been acquired according to the projection matrix and the coordinate transform. Afterwards,the position and the shape of structured light can be calibrated by means of multi-point fitting. Finally,the feasibility and validity of this method have been confirmed by the experiment which involves the calibration of the nine structured lights in the 3-D binocular measurement system based on the multi-stripes projection.