The continuous light coming from the Ar + laser can be converted to pulse light when an optical chopper is equipped.The sample is excited by the pulse light in the confocal microscopy system.The light intensity of the excitation light and the phosphorescence is simultaneously recorded in terms of gray value by the confocal microscopy system.The phosphorescence lifetime measurement can be realized with time resolved analysis for the phosphorescence intensity decay.The different decay lifetimes of the Oxy Phor R2 sample under different oxygen concentration are measured with the reconstructed confocal microscopy system.