| 杨语谌,张威,周连群,姚佳,郭振,张芷齐,严茹红,朱红楠.家用aPTT检测技术芯片修饰方法的研究[J].光电子激光,2018,29(1):57~63 |
| 家用aPTT检测技术芯片修饰方法的研究 |
| Study on the method of modifying domestic aPTT detection technology chip |
| 投稿时间：2017-04-28 |
| DOI： |
| 中文关键词: 活化部分凝血活酶时间(aPTT)检测 压电石英晶体微天平(QCM) Parylene-C修饰 稳 定性 重复性 |
| 英文关键词:activated partial thromboplastin time (aPTT) detection quartz crystal microbala nce (QCM) Parylene-C modification stability repeatability |
| 基金项目:国家自然科学基金(51675517)、江苏省自然科学基金(BK20140380,BK20160057)、中科院青年创新促进会(2014280)、江苏省产业技术研究院重大原始创新(B020155007)和中国博士后科学基金(2016M601890)资助项目 (1.苏州生物医学工程技术研究所,江苏 苏州 215163; 2.中国科学院大学,北京 100049; 3.苏州科技城医院检验科,江苏 苏州 215153) |
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| 中文摘要: |
| 采用凝血检测光学透射比浊方法检测活化部分凝血活酶时间(aPTT), 尝试用聚乙烯(PE)、聚苯乙烯(PS)和Parylene-C 3种疏水材料, 研究压电石英晶体微电平(QCM)传感芯片表面修饰。 修饰后,芯片品质用网络分析仪衡量,PE、PS和Parylene-C 3种疏水材料的疏水修饰接触角分别为92.3°、90.7°和93.3°。结果表 明,Parylene-C修饰后QCM芯片的品质提高,有效信号峰-峰值提高了11%；用于 光学aPTT检测 对比体现出良好重复性,血浆7次检测变异系数(CV)为1.51%, 重复性良 好,全血7次检测CV为1.15%。本文的Pary lene-C修饰QCM,可为家用aPTT检测仪器的开发提供参考。 |
| 英文摘要: |
| The optical transmission turbidimetry is used to detect activated partial thromb oplastin time (aPTT),and the surface modification of quartz crystal microbalance (QCM) is studied by using three kind s of materials,which include polyethylene (PE),polystyrene (PS) and Parylene- C.Three kinds of modified QCM (PE-QCM,PS-QCM and P-QCM) were evaluated by network analyzer.In addition,h ydrophobic contact angles are 92.3°,90.7° and 93.3°,respectively.The result s show that the quality of P-QCM device is improved,the effective signal pea k to peak value is increased by 11%,the comparison of optical aPTT detection shows good re peatability,plasma 7times detection coefficient of variation (CV) is 1.51%,and 7w hole blood tests CV is 1.15%.In a conclusion,Parylene-C modified QCM provides key technology of inst rument on domestic aPTT testing. |
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