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彭义杰,刘木华,赵进辉,袁海超,李耀,陶进江,郭红青.基于纳米Au胶表面增强拉曼光谱对水中链霉素残留量的快速检测[J].光电子激光,2016,27(8):845~852

基于纳米Au胶表面增强拉曼光谱对水中链霉素残留量的快速检测

Rapid determination of streptomycin residues in water using surface enhanced Ram an spectroscopy with Au nanoparticles

投稿时间：2015-11-16

DOI：

中文关键词: 表面增强拉曼光谱(SERS) 硫酸链霉素(STR) Au胶纳米颗粒快速检测

英文关键词:surface enhanced Raman spectroscopy (SERS) streptomycin sufate (STR) Au nanopa rticles rapid determination

基金项目:国家自然科学基金(31101295)、江西省科技厅对外科技合作计划(20132BDH80005)和江西省科技厅科技支撑(2012BBG70058)资助项目

作者	单位
彭义杰	江西农业大学工学院生物光电及应用重点实验室,江西南昌 330045
刘木华	江西农业大学工学院生物光电及应用重点实验室,江西南昌 330045
赵进辉	江西农业大学工学院生物光电及应用重点实验室,江西南昌 330045
袁海超	江西农业大学工学院生物光电及应用重点实验室,江西南昌 330045
李耀	江西农业大学工学院生物光电及应用重点实验室,江西南昌 330045
陶进江	江西农业大学工学院生物光电及应用重点实验室,江西南昌 330045
郭红青	江西农业大学工学院生物光电及应用重点实验室,江西南昌 330045

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中文摘要:

为实现快速检测水中硫酸链霉素(STR)的残留,探索建立一种STR水溶液的表面增强拉曼光谱(SERS,surface enhanced Raman spectroscopy)检测方法。应用电磁炉加热以柠檬酸三钠还原氯金酸(HAucl ₄)制成的Au胶纳米颗粒作为表面增强基底,先后分析了Au胶纳米颗粒的吸收光谱、STR水溶液的SERS以及拉曼峰归属；然后确定了最佳的光谱仪功率、Au胶纳米颗粒的加入量和吸附时间；最后以1031cm－1处拉曼峰作为特征峰建立STR水溶液的标定曲线,并对标定曲线的准确度和精确度进行验证。实验结果表明,当水中STR质量浓度范围为2.0~20.0 mg/L时,1031 cm－1处的拉曼峰强度与STR水溶液的质量浓度有良好的线性关系, 线性方程为Y=293.31X+435.42, 决定系数R²为0.933 。真实值与预测值之间的R²为0.964,均方根误差(RMSEP)为1.3043mg/L,回收率为92.1~133.0%,相对标准偏差(RSD,n=5)为0.88~2.41%。

英文摘要:

A new method using surface enhanced Raman spectroscopy (SERS) was established f or the rapid determination of streptomycin residues in water.The Au nanoparticles,which were prepared via sodium citrate deoxidizing tetrachloroaura tetrihydrate with electromagnetic heating,were used as SERS substrate.Firstly,the ab sorption spectra of the Au nanoparticles,the SERS spectra of streptomycin solution and its vibrat ional assignment were analyzed.And then the spectrometer′s power,the addition amount of Au nanop articles and the adsorption time were determined,respectively.Finally,the 1031cm－1 was used as the characteristic peak to establish the standard curve of streptomycin solution,and the precision and a ccuracy of the standard curve were verified.The results show that a good linearity is obtain ed between the peak intensity at 1031cm－1 and streptomycin solution concentration (2.0－20.0mg/L),the linear equation is Y=293.31X+435.42,and the determination coeff icient is 0.9337.The determination coefficient of streptomycin solutions between the actual values and the predicti ve values is 0.9643,and the root mean square error (RMSEP) is 1.3043mg/L.Recovery rate of streptomycin residues in water is 92.1%－133.0%,and the relative standard deviation is betwe en 0.88% and 2.41% (n=5).It is feasible that the Au nanoparticles ar e used as the surface substrate and the Raman spectroscopy is used for the rapid determination of streptomycin residues in water.

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